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lab tek-ii chamber slides  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lab tek-ii chamber slides
    Lab Tek Ii Chamber Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lab+tek+ii+chamber+slides/8+well+chamber+slides/pmc12273755-499-7-11
    Average 90 stars, based on 1 article reviews
    lab tek-ii chamber slides - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Expression of ADAM15 in rheumatoid synovium: up-regulation by vascular endothelial growth factor and possible implications for angiogenesis
    Article Snippet: Human umbilical vein endothelial cells (HUVECs 7943; Cambrex Co., East Rutherford, NJ, USA) were grown in medium EBM-2 supplemented with EGM-2 (Cambrex Co.). .. In order to examine the expression of VEGFR-2 and ADAM15 and exclude the possibility of contamination of cultured RA SF by endothelial cells, RA SFs at 5–9 passages were seeded on Lab-Tek II chamber slides (Nalge-Nunc International, Naperville, IL, USA) and subjected to immunohistochemistry for VEGFR-2, ADAM15, CD31 and vWF as described above. ..

    Article Title: FET fusion oncoproteins disrupt physiologic DNA repair networks and induce ATR synthetic lethality in cancer
    Article Snippet: .. Briefly, U20S cells expressing EWS-GFP, EWS-FLI1-GFP, or EWS-ATF1-GFP were seeded in 8-well Lab Tek II Chamber Slides (Thermo Fisher Scientific) for 24 hours. .. Cells were treated with 1μg/ml Hoechst 33342 (Thermo Fisher Scientific) for 30 minutes prior to micro-irradiation.

    Cell Culture:

    Article Title: Expression of ADAM15 in rheumatoid synovium: up-regulation by vascular endothelial growth factor and possible implications for angiogenesis
    Article Snippet: Human umbilical vein endothelial cells (HUVECs 7943; Cambrex Co., East Rutherford, NJ, USA) were grown in medium EBM-2 supplemented with EGM-2 (Cambrex Co.). .. In order to examine the expression of VEGFR-2 and ADAM15 and exclude the possibility of contamination of cultured RA SF by endothelial cells, RA SFs at 5–9 passages were seeded on Lab-Tek II chamber slides (Nalge-Nunc International, Naperville, IL, USA) and subjected to immunohistochemistry for VEGFR-2, ADAM15, CD31 and vWF as described above. ..

    Article Title: Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells
    Article Snippet: .. For immunocytochemistry, GCs were isolated as described above and cultured in Lab Tek II Chamber Slides (Thermo Fisher Scientific, Waltham, MA) for 24 h before treatment with FSH (50 ng/mL) for 1 h. Then, cells were fixed with 4% paraformaldehyde in PBS for 10 min at room temperature and permeabilized with 1% Triton X-100 and stained using ImmPACT SG Substrate, Peroxidase (Vector Laboratories) following manufacturer’s instructions. .. Short hairpin RNAs (shRNAs) under the control of the U6 promoter were used to specifically knock down the expression of GATA4. shRNA target recognition sequences used were shGATA4: GGA TTT AAT TCG TAT ATA T; and shLUC (control): GCC TGA AGT CTC TGA TTA AGT ACA A. Oligonucleotides and their corresponding antisense sequence separated by a short spacer sequence were chemically synthesized (Integrated DNA Technologies, Inc., Coralville, IA).

    Article Title: The signaling cascade of induction and maintenance of ES cell diapause
    Article Snippet: .. STED imaging of BRD4 ES cells were cultured and plated on Nunc Lab Tek II Chamber Slides (Thermo Scienti c, 154534PK). .. Cells were xed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in DPBS for 15 minutes at RT and then washed twice with DPBS.

    Immunohistochemistry:

    Article Title: Expression of ADAM15 in rheumatoid synovium: up-regulation by vascular endothelial growth factor and possible implications for angiogenesis
    Article Snippet: Human umbilical vein endothelial cells (HUVECs 7943; Cambrex Co., East Rutherford, NJ, USA) were grown in medium EBM-2 supplemented with EGM-2 (Cambrex Co.). .. In order to examine the expression of VEGFR-2 and ADAM15 and exclude the possibility of contamination of cultured RA SF by endothelial cells, RA SFs at 5–9 passages were seeded on Lab-Tek II chamber slides (Nalge-Nunc International, Naperville, IL, USA) and subjected to immunohistochemistry for VEGFR-2, ADAM15, CD31 and vWF as described above. ..

    Immunocytochemistry:

    Article Title: Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells
    Article Snippet: .. For immunocytochemistry, GCs were isolated as described above and cultured in Lab Tek II Chamber Slides (Thermo Fisher Scientific, Waltham, MA) for 24 h before treatment with FSH (50 ng/mL) for 1 h. Then, cells were fixed with 4% paraformaldehyde in PBS for 10 min at room temperature and permeabilized with 1% Triton X-100 and stained using ImmPACT SG Substrate, Peroxidase (Vector Laboratories) following manufacturer’s instructions. .. Short hairpin RNAs (shRNAs) under the control of the U6 promoter were used to specifically knock down the expression of GATA4. shRNA target recognition sequences used were shGATA4: GGA TTT AAT TCG TAT ATA T; and shLUC (control): GCC TGA AGT CTC TGA TTA AGT ACA A. Oligonucleotides and their corresponding antisense sequence separated by a short spacer sequence were chemically synthesized (Integrated DNA Technologies, Inc., Coralville, IA).

    Isolation:

    Article Title: Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells
    Article Snippet: .. For immunocytochemistry, GCs were isolated as described above and cultured in Lab Tek II Chamber Slides (Thermo Fisher Scientific, Waltham, MA) for 24 h before treatment with FSH (50 ng/mL) for 1 h. Then, cells were fixed with 4% paraformaldehyde in PBS for 10 min at room temperature and permeabilized with 1% Triton X-100 and stained using ImmPACT SG Substrate, Peroxidase (Vector Laboratories) following manufacturer’s instructions. .. Short hairpin RNAs (shRNAs) under the control of the U6 promoter were used to specifically knock down the expression of GATA4. shRNA target recognition sequences used were shGATA4: GGA TTT AAT TCG TAT ATA T; and shLUC (control): GCC TGA AGT CTC TGA TTA AGT ACA A. Oligonucleotides and their corresponding antisense sequence separated by a short spacer sequence were chemically synthesized (Integrated DNA Technologies, Inc., Coralville, IA).

    Staining:

    Article Title: Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells
    Article Snippet: .. For immunocytochemistry, GCs were isolated as described above and cultured in Lab Tek II Chamber Slides (Thermo Fisher Scientific, Waltham, MA) for 24 h before treatment with FSH (50 ng/mL) for 1 h. Then, cells were fixed with 4% paraformaldehyde in PBS for 10 min at room temperature and permeabilized with 1% Triton X-100 and stained using ImmPACT SG Substrate, Peroxidase (Vector Laboratories) following manufacturer’s instructions. .. Short hairpin RNAs (shRNAs) under the control of the U6 promoter were used to specifically knock down the expression of GATA4. shRNA target recognition sequences used were shGATA4: GGA TTT AAT TCG TAT ATA T; and shLUC (control): GCC TGA AGT CTC TGA TTA AGT ACA A. Oligonucleotides and their corresponding antisense sequence separated by a short spacer sequence were chemically synthesized (Integrated DNA Technologies, Inc., Coralville, IA).

    Article Title: A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells
    Article Snippet: .. Hoechst 33342 staining LN-229 cells were seeded into LAB-TEK ® II chamber slides (Nalge Nunc International, Rochester, NY) at 5 × 10 3 cells per chamber and incubated overnight before subjected to 0, 10, 50, and 100 μg/mL of PM 2.5 exposure for 24 h. The cells were washed three times with 1x PBS before fixing with 10% formalin for 30 min at RT. .. After washed with 1x PBS, the cells were stained with 1 μg/mL of Hoechst 33342 (Thermo Scientific, Rockford, IL) for 15 min in dark.

    other:

    Article Title: Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone
    Article Snippet: Cells were cultured in Lab-TekTMII Chamber Slides (Thermo Scientific, 154453) for 72 h in complete media.

    Imaging:

    Article Title: The signaling cascade of induction and maintenance of ES cell diapause
    Article Snippet: .. STED imaging of BRD4 ES cells were cultured and plated on Nunc Lab Tek II Chamber Slides (Thermo Scienti c, 154534PK). .. Cells were xed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in DPBS for 15 minutes at RT and then washed twice with DPBS.

    Incubation:

    Article Title: A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells
    Article Snippet: .. Hoechst 33342 staining LN-229 cells were seeded into LAB-TEK ® II chamber slides (Nalge Nunc International, Rochester, NY) at 5 × 10 3 cells per chamber and incubated overnight before subjected to 0, 10, 50, and 100 μg/mL of PM 2.5 exposure for 24 h. The cells were washed three times with 1x PBS before fixing with 10% formalin for 30 min at RT. .. After washed with 1x PBS, the cells were stained with 1 μg/mL of Hoechst 33342 (Thermo Scientific, Rockford, IL) for 15 min in dark.



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